P9182-02A-BIOTIN
PTEN Tumor Suppressor Protein Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1) (Biotin)
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£914.00
- SKU:
- P9182-02A-BIOTIN
- Reinheit:
- Purified by Protein A chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein derived from the PTEN protein.
- Klon:
- 2F4C9
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Weitere Details:
- PTEN (also know as MMAC1) was recently identified as a candidate tumor suppressor gene. Deletions or mutations within the PTEN gene have been observed in a variety of tumor cell lines and cancer cell types. Based on PTEN’s structural features, the protein was originally thought to be a protein tyrosine phosphatase. However, recent evidence indicates that PTEN is actually a lipid phosphatase rather that a protein tyrosine phosphatase. In fact, the target substrate for PTEN is phosphatidylinositol-3,4,5-triphosphate (Ptd-Ins(3,4,5)P3). Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control. Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands. By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to “turn off” the Ptd-Ins(3,4,5)P3 pathway and there by suppress cellular proliferation. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml ELISA: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice