P9109-11
Protein Tyrosine Phosphatase 1B (PTP1B)
Größe
£837.00
- SKU:
- P9109-11
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant protein corresponding to Glu2-Thr435 from human PTP1B.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Protein tyrosine phosphatase 1B (PTP1B), also known as protein tyrosine phosphatase, non-receptor type 1 (PTPN1) and placental protein tyrosine phosphatase, dephosphorylates tyrosine residues in proteins. PTP1B is a cytosolic enzyme anchored in the endoplasmic reticulum that plays a critical role in regulating growth factor responsiveness. PTP1B knockout mice show increased resistance to high fat diet-induced obesity and have exaggerated responses to insulin. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Knockout Validated: PTP1B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PTP1B knockout HeLa cell line. Western Blot: 0.05-0.3ug/ml using TF‑1 human erythroleukemic cell line and CHP‑100 human neuroblastoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice