P9108-09D
Protein S (PROS1, PROS, Vitamin K Dependent Protein S) (Not for Export EU)
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£594.00
- SKU:
- P9108-09D
- Reinheit:
- Serum
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Human Protein S
- Format:
- Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
- Weitere Details:
- Protein S is a vitamin K dependent plasma glycoprotein (MW 69000) which belongs to a group of coagulation inhibitors consisting itself of proteinases. It circulates in the blood in a free active form and in an inactive form bound to C4b binding protein. Protein S is also released by thrombin stimulation from platelet alpha-granules. Protein S acts a s a cofactor for activated protein C (pCa) enhancing the cleavage of factors Va and VIIIa, producing anticoagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system. Protein S looses much of its coagulant cofactor activity after cleavage by thrombin, a process which is inhibited by thrombomodulin in the presence of free calcium ions. Plasma concentration of protein S may be significantly reduced below normal adult levels (2.5mg/ml) in patients with hereditary deficiency of protein S or suffering a combination of deep vein thrombosis, superficial thrombophlebitis and pulmonary embolism. Substances such as bacterial endotoxins, tumor necrosis factor and immune mediator interleukin 1 also reduce the protein S level in plasma. Oral contraceptives also depress pS activity. A reduction in plasma protein S during pregnancy usually returns to normal after parturition. Applications: Suitable for use in ELISA, Immunoprecipitation, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul human serum Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wells. Note: Plasma samples and all assay components must contain EDTA to stabilize the protein. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice