P9107-20J
Protein Phosphatase 1G (formerly 2C), Magnesium-dependent, gamma (Protein Phosphatase Magnesium-dependent 1 gamma, PPM1G, MGC1675, MGC2870, Protein Phosphatase 1C, Protein Phosphatase 2 Catalytic Subunit gamma Isoform, Protein phosphatase 2C gamma isoform, PP2C gamma, PP2CG, PPP2CG)
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£761.00
- SKU:
- P9107-20J
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli-derived recombinant protein corresponding to Met1-Asp546 from human PP2Cgamma.
- Klon:
- 9H174
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- Protein phosphatase 2C isoform gamma (PP2C gamma; also PPM1G) is a widely expressed, 75kD member of the PP2C family of Ser/Thr protein phosphatases. Human PP2C gamma is 546 amino acids (aa) in length. The apparent mass is much larger than the predicted mass of 59kD because of the presence of an acidic region (aa 259-326) rich in glutamate and aspartate residues, causing the protein to move anomalously on SDS-PAGE. The protein contains numerous sites for phosphorylation by casein kinase II, particularly within the acidic domain. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml Immunohistochemistry: 8-25ug/ml with immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue. IOptimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice