P9103-72Y
Protein Kinase, cAMP-dependent, Regulatory, Type I, alpha (PRKAR1A, CNC1, DKFZp779L0468, MGC17251, PKR1, PPNAD1, PRKAR1, Tissue Specific Extinguisher 1, TSE1)
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£448.00
- SKU:
- P9103-72Y
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide near the N-terminus of human PRKAR1A
- Klon:
- 8C274
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
- Weitere Details:
- cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in this gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. Three alternatively spliced transcript variants encoding the same protein have been observed. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice