P9103-59N
Protein Kinase C, mu, Protein Kinase D, phosphorylated (Ser916) (PKCm, PKD)
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£601.00
- SKU:
- P9103-59N
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser916 of mouse PKD.
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Weitere Details:
- Activation of PKC is one of the earliest events in the cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCmu, is a serine/threonine kinase (3,4). Activation of PKD/PKCmu is dependent on the phosphorylation of two activation loop sites, namely serine 744 and serine 748, via a PKC-dependent signal transduction pathway (5). In addition to the two activation loop sites, the C-terminal serine 916 has been identified as an autophosphorylation site for PKD/ PKCmu. Phosphorylation at the Ser916 site correlates with PKD/PKCmu catalytic activity (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice