P9102-95R

Protein Kinase G1 beta, N-terminal HIS-Tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase)

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£912.00

SKU:: P9102-95R
Molekulargewicht:: 78
Reinheit:: Purified ~70%
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.
Weitere Details:: Source: Recombinant corresponding to full length human PKG1 beta with N-terminal His tag expressed in Baculovirus infected Sf9 cell expression system (NM_006258). Specific Activity: 0.85pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (SER/THR 14) at RT for 1hour. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.