P9099-02E
Proteinase 3 (Proteinase-3, PR3, PR-3, PRTN3, ACPA, Azurophil Granule Protein 7, AGP7 Serine Proteinase, AGP7, cANCA, c-ANCA Antigen, EC 3.4.21.76, Leukocyte Proteinase 3, MBT Wegener Autoantigen, MBT, Myeloblastin, MBN, Neutrophil Proteinase 4, NP-4, p29, Serine Proteinase Neutrophil Wegener Granulomatosis Autoantigen, Wegener Autoantigen) (Not for Export EU)
Größe
£607.00
- SKU:
- P9099-02E
- Reinheit:
- Whole serum
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Human leucocyte proteinase 3.
- Format:
- Supplied as a lyophilized powder. Reconstitute with 250ul of sterile ddH2O. For long term storage, a preservative may be added.
- Weitere Details:
- Proteinase 3 (PR3), a constituent of azurophil granules of neutrophils (polymorphonuclear cells, PMNs), is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA) in Wegener’s Granulomatosis (WG). Applications: Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000 -1:2000 Western Blot: 1:200-1:500 Immunoprecipitation: 1:100 -1:200 Immunohistochemistry: 1:50 -1:100 Immunocytochemistry: 1:50- 1:100 Optimal dilutions to be determined by the researcher Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice