Prostaglandin Dehydrogenase 1 (HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), Prostaglandin dehydrogenase 1)

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£642.00

SKU:: P9053-27A
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: C-DYDTTPFQAKTQ, C Terminus
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: Alternate Name: HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), Prostaglandin dehydrogenase 1 Domain Information: Gene Ontology Terms: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; electron transporter activity; metabolism; oxidoreductase activity; prostaglandin metabolism; prostaglandin-D synthase activity Application(s): Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: experiments gave bands at approx 26kD and 50kD in Human Duodenum lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature (PMID: 11889207). This protein has a calculated MW of 29.0kD according to NP_000851. Recommended concentration: 0.1-1ug/ml. 0.1ug/ml staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See P9053-27A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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