P9004-28
PRMT5 (Protein arginine N Methyltransferase 5, Shk1 Kinase-binding Protein 1 Homolog, Jak-binding Protein 1, JBP1)
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£537.00
- SKU:
- P9004-28
- Anwendung:
- WB
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptides corresponding to human PRMT5 amino acid sequence.
- Format:
- Supplied as a liquid in 200ul of PBS containing 0.02% sodium azide and 0.1 mg/ml BSA.
- Weitere Details:
- Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1,000 HeLa cell lysate can be used as positive control. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice