P4700-05

Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

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£787.00

SKU:: P4700-05
Reinheit:: Purified by Protein G affinity chromatography from ascites.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to rat PSD-95
Klon:: 6G6-1C9
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Weitere Details:: Postsynaptic density protein 95kD (PSD-95), also known as Synapse associated protein 90kD (SAP90), is a brain specific protein that is highly similar to the Drosophila dlg tumor suppressor protein (1). PSD-95 is a member of membrane associated proteins that are localized beneath the postsynaptic membrane of synapses in the CNS. PSD-95 contains a carboxyl-terminal guanylate kinase domain, an upstream SH3 domain, and three amino-terminal PDZ domains. PSD-95 interacts with NMDA receptor and Shaker-type K+ channel and contributes to their clustering and localization at the synaptic spines in hippocampal neurons and the pinceau terminal of cerebellar basket cells, respectively (2). The yeast two hybrid method revealed that the second PDZ domain in PSD-95 binded tightly to the carboxyl terminal (t)-S/TXV sequence of the NR2B subunit of NMDA receptor and of Shaker-type K+ channel. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interaction between PDZ domains present on both proteins. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot (ECL): 5ug/ml Immunoctyochemistry: 12.5ug/ml Optimal dilutions to be determined by researcher. Positive Controls: Mouse Brain Tissue Extract Rat Brain Tissue Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice