P4275-07B

Plk3, Recombinant, Human, N-terminal His-Tag, Serine Threonine Kinase, aa 58-339 (CNK, FNK, PRK, Polo-like kinase 3)

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£912.00

SKU:: P4275-07B
Molekulargewicht:: 36.7
Reinheit:: ≥ 90% by SDS-PAGE
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.
Weitere Details:: Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal HIS6 tag, MW 36.7kD, expressed in an Baculovirus infected Sf9 cell expression system. Purity: >80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of casein substrate per minute at pH 7.4 and 30°C Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 2mg/ml casein, 0.1mM ATP and 0.1ug/ml Plk3. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.