P4275-06

Plk2, Recombinant, Human, GST-Tag (SNK, Polo-like kinase 2)

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£912.00

SKU:: P4275-06
Molekulargewicht:: 107
Reinheit:: ≥ 70% by SDS-PAGE
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol and 3mM DTT.
Weitere Details:: Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST-HIS6 tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay Buffer: 10mM HEPES, pH 7.4, 5mM MgCl2, 10uM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.4mM cold ATP/ 2uCi [g-32P]ATP and 0.5ug/ul alpha-casein substrate. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.