P4186-90

Pin1 (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1)

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£800.00

SKU:: P4186-90
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Full-length His tagged human Pin1.
Format:: Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide, before the addition of glycerol to 30%.
Weitere Details:: Pin1 is a peptidyl-prolyl isomerase which is present throughout the cell cycle and which is thought to alter its mitotic targets by changing their conformation. Many of the substrates of the mitotic regulator PIN1 are recognized by the monoclonal antibody, clone MPM-2. This clone recognizes a subset of M phase phosphoproteins in a phosphorylation-dependent manner. It has also been shown that RNA polyermase II large subunit interacts with PIN1 in HeLa cells and may thus regulate transcriptional and post-transcriptional events by catalyzing phosphorylation-dependent conformational changes. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ul. Detected Pin1 in RIPA lysates from rat PC-12 and human HeLa cells. Immunoprecipitation: 4ug. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Positive Antigen Control Included: F4209: Fibroblast Cell, 3T3/A31. Add 2.5ul of 2-mercaptoethanol for each 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ul of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice