P4074-06D5
Phospholipase A2, Secretory, Group IB (PLA2G1B, phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 precursor, PLA2, PLA2A, PPLA2)
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£780.00
- SKU:
- P4074-06D5
- Reinheit:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Ser148 from human PLA2G1B expressed in NSO cells.
- Klon:
- 11n151
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- PLA2G1B, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78-76aa sequence identity with mouse and rat PLA2G1B, respectively. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using human pancreas tissue under non-reducing conditions Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human PLA2G1B. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice