P4073-33J

Phosphoinositide 3 Kinase, p110 beta (p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Polypeptide, PI3-kinase p110 Subunit beta, PI3K, PI3Kbeta, PI3KCB, PIK3C1, PtdIns-3-kinase p110)

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£761.00

SKU:: P4073-33J
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110 beta, conjugated to KLH. Species Sequence Homology: Monkey, canine, Rat and Mouse.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival, signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85kD regulatory subunit and a 110kD catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospholipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the beta:gamma subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.5ng/well Western Blot: 0.05-0.1ug/ml Immunocytochemistry: 1:100 Optimal dilutions to be determined by the researcher. Positive Control: HEK-293 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice