P4071-16A
Phosphatase, Alkaline, Placental (Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, ALP, FLJ61142, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1)
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£435.00
- SKU:
- P4071-16A
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Placental membrane.
- Klon:
- 3H2834 (H17E2)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Weitere Details:
- Human placental alkaline phosphatase is also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535aa ~67kD GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982) and in ovarian carcinoma (Tholander et al. 1990). P4071-16A has been widely used in immunolocalization studies of germ cell tumors and may also be used in ELISA based assays for the measurement of soluble PLAP. Applications: Suitable for use in Immunohistochemistry, ELISA and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ug/ml. 10ul labels 10e6 cells in 100ul. Immunohistochemistry: Frozen sections. Reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation is recommended. Not suitable for use on paraffin sections. Optimal dilutions to be determined by the researcher. Recommended Immunohistology Positive Control: Placenta, seminoma Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Recommended Secondary Antibodies: I1904-65V: IgG, F(ab’)2 (FITC) Pab RbxMo I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab RbxMo I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice