P4071-16A-BIOTIN
Phosphatase, Alkaline, Placental (Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, ALP, FLJ61142, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1) (Biotin)
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£960.00
- SKU:
- P4071-16A-BIOTIN
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Placental membrane.
- Klon:
- 3H2834 (H17E2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- Human placental alkaline phosphatase is also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982) and in ovarian carcinoma (Tholander et al. 1990). P4071-16A has been widely used in immunolocalization studies of germ cell tumors and may also be used in ELISA based assays for the measurement of soluble PLAP. Applications: Suitable for use in Immunohistochemistry, ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections. Reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation is recommended; Not suitable for use on paraffin sections. Optimal dilutions to be determined by the researcher. Recommended Immunohistology Positive Control: Placenta, seminoma Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice