P4071-10W
Phosphatase, Alkaline, Calf Intestine, Solution, 4000U/mg Protein
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£557.00
- SKU:
- P4071-10W
- Molekulargewicht:
- 110150
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as liquid, crystalline suspension in 70% ammonium sulfate. Free of endonuclease, exonuclease and RNase activities. No preservatives added.
- Weitere Details:
- Native calf alkaline phosphatase. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: May be diluted in sterile ddH2O or in dilute buffer. Optimal dilutions to be determined by the researcher. Activity: 4000U/mg protein. 1 unit liberates 1umole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-notrophenyl phosphate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice