P3360-07
PF4 (Platelet Factor 4)
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£416.00
- SKU:
- P3360-07
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human PF4 derived from E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Weitere Details:
- Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1-2ug/ml with an overnight incubation at 4ºC. An AP-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with sodium citrate, pH 6, is reccommended. ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human PF4 (P3360A) as a detection antibody, allows the detection of at least 2000-4000pg/ml of recombinant human PF4 Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human PF4 is 1.5-3ng/lane under reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
- Versandbedingungen:
- Blue Ice