Pericentrin 1 (PCNT1, PCN, PCNT, Pericentrin 1, Frount, Nucleoporin Nup75, Nucleoporin 85kD, NUP85)

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£642.00

SKU:: P3340-50
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to sequence C-ARAIIREGSLEGS from the C-terminal of human pericentrin 1 (NP_079120). Species sequence homology: mouse and rat
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: Pericentrin 1 is a 75kD component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localised protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Applications: Suitable for use in Immunoprecipitation, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000 Western Blot: 1-3ug/ml, ~75kD band observed in HeLa lysates (calculated MW of 75kD according to NP_079120) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice