P3140-04
PEDF R (Pigment Epithelial Derived Factor Receptor, Patatin-like Phospholipase Domain-containing Protein 2, PEDFR, PNPLA2, Desnutrin)
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£819.00
- SKU:
- P3140-04
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Val162-Gly253 from mouse PEDF R, expressed in E. coli.
- Klon:
- 11D53
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55kD member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486aa type II transmembrane (TM) protein. Its TM segment is stated to span (aa9-29). Alternatively, it may have a hydrophobic lipid-association domain between (aa267-295). It contains a palatin domain (aa10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of (aa163-196) and (aa253-308), respectively. Over (aa162-253), mouse PEDF R shares 98% and 93% aa identity with rat and human PEDF R, respectively. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml using mouse adipose tissue lysates Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice