PED/PEA-15, phosphorylated (Ser104)

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£639.00

SKU:: P3139-85A
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phospho-peptide corresponding to residues surrounding Ser104 of human PEA-15. Species Sequence Homology: mouse
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Weitere Details:: PEA-15 is a 15kD phosphoprotein expressed abundantly in astrocytes, fibroblasts and tissues including lung and eye. The protein has been shown to coordinate cell growth, death and glucose ultilization. The amino-terminal DED domain of PEA-15 mediates its binding to FADD or ERK and futher regulates the ERK and apoptosis pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to ERK and FADD. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.