P3127-05A
Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensitive Factor, GSF, Insulin Promoter Factor 1, IPF1, Islet/Duodenum Homeobox 1, IDX1, Insulin Upstream Factor 1, IUF1, MODY4, Somatostatin Transactivating Factor 1, STF1)
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£856.00
- SKU:
- P3127-05A
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Met1-Arg284 of mouse Pdx1 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42kD member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta-cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta-cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284aa in length (Accession # P52946). It contains one transactivation domain (aa13-73) a PBX1 P-P-M-W-K dimerization motif (aa119-124), and a DNA-binding homeodomain with an embedded NLS (aa148-206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistocehmistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml beta TC-6 mouse beta cell insulinoma cell line Immunocytochemistry: 5-15ug/ml Immersion fixed beta TC-6 mouse beta cell insulinoma cell line Immunohistocehmistry: 5-15ug/ml Perfusion fixed frozen sections of mouse pancreas Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice