P3113-82C4
Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1, Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1)
Größe
£672.00
- SKU:
- P3113-82C4
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1, conjugated to KLH.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Weitere Details:
- Patched 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml detected Patched 1 on 10ug of human skeletal muscle, fetal tissue lysate. Immunohistochemistry (Paraffin): 1:300 detected Patched 1 in kidney tissue. Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Positive Control: Human skeletal muscle, fetal tissue lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice