P3113-06D
PARP, cleaved (Poly ADP-Ribose Polymerase, ADP Ribosyltransferase NAD+, ADPRT, ADPRT1, NAD(+) ADP Ribosyltransferase 1, pADPRT-1, Poly Adenosine Diphosphate ADP Ribose Polymerase, Poly [ADP-ribose] Polymerase 1, PARP1, PARP-1, Poly (ADP-ribose) Polymerase Family Member 1, Poly [ADP-ribose] Synthetase 1, PPOL)
Größe
£581.00
- SKU:
- P3113-06D
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids near 214/215-cleavage site of human PARP.
- Klon:
- 9A291 (194C1439)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Weitere Details:
- The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kD nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kD fragment containing the DNA binding domain and an 89kD fragment containing the catalytic and automodification domains. The 24kD-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Flow Cytometry (intracellular): 1-10ug/1x10e6 cells Optimal dilutions to be determined by the researcher. Positive Control: Camptothecin treated HL-60 (12 hr) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice