P2488-15C
PAK1,2,3, phosphorylated (Thr423/Thr402/Thr436) (p21 Activated Kinase 1,2,3)
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£914.00
- SKU:
- P2488-15C
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Human PAK 1/2/3 phospho-peptide corresponding to a region of the human protein conjugated to KLH. Sequence Information: PEQSKRS-(pT)-MVGTPY (aa416-429 of human PAK1, aa395-408 of human PAK2 and aa429-444 of human PAK3)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
- Weitere Details:
- The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis. Mammalian PAKs are called 1, 2, 3 or a, g, b respectively. PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae. Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids. PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins. The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution(s): ELISA: 1:5000-1:25,000; Assayed against 0.1ug of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Positive Control: Lysates from Jurkat cells or PAK transfected cells may be used as a control. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice