P2150-50B
Paired Immunoglobulin-Like Type 2 Receptor beta (PILRb) (Biotin)
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£908.00
- SKU:
- P2150-50B
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- NS0-derived recombinant protein corresponding to Gln20-Ala189 from human Paired lgG-Like Type 2 Receptor beta.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Weitere Details:
- Paired immunoglobulin-like, type 2 receptor beta (PILR-b) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. It is the activating counterpart to the ITIM-bearing PILR-a inhibitory receptor. PILR-b is expressed in a wide variety of tissues including hematopoietic cells. Mature human PILR-b is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and positively-charged residues in its TM domain that interacts with ITAM-bearing adaptor molecules. Within the V-type Ig-like region in their ECD, human PILR-b and PILR-a share a 92% aa sequence identity. The aa sequence of mouse PILR-b ECD is only 43% identical to that of the human protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2 ug/mL detection limit for rhPILR-b ~ 1ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice