Paired Immunoglobulin-Like Type 2 Receptor beta (PILRb)

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£908.00

SKU:: P2150-50
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Gln20-Ala189 of human Paired Immunoglobulin-Like Type 2 Receptor beta expressed in NS0 cell line.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: Paired immunoglobulin-like, type 2 receptor beta (PILR-beta) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. It is the activating counterpart to the ITIM-bearing PILR-alpha inhibitory receptor. PILR-beta is expressed in a wide variety of tissues including hematopoietic cells. Mature human PILR-beta is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and positively-charged residues in its TM domain that interacts with ITAM-bearing adaptor molecules. Within the V-type Ig-like region in their ECD, human PILR-beta and PILR-alpha share a 92% aa sequence identity. The aa sequence of mouse PILR-beta ECD is only 43% identical to that of the human protein. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. In this format, this antibody shows ~10% cross-reactivity with recombinant human PILR-alpha and <5% cross-reactivity with recombinant mouse PILR-beta, PILR-alpha, and PILR-L. Direct ELISA: 0.5-1ug/ml. The detection limit for is ~0.3ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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