P1001-97B
p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein)
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£843.00
- SKU:
- P1001-97B
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant human p130Cas, Met66-Val412
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- p130Cas (protein 130kD Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93kD, it runs anomalously at 105-130kD in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31kD fragment promotes anoikis and cell death. Human p130Cas is 870aa in length. It contains one SH3 domain (aa3-65), a Pro-rich region (aa74-87), at least 10 phosphotyrosine (aa128-410) and four phosphoserine (aa134-639) sites, an SH3 binding motif (aa635-643) and a HLH dimerization segment that interacts with CASL. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice