P1001-26H-ML490

p53, aa181-190 (Tumor Suppressor Protein, Oncogene Protein) (MaxLight 490)

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£769.00

SKU:: P1001-26H-ML490
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human p53.
Klon:: 3H2809
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Weitere Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line. Recommended Positive Control Tissue: Colon or breast carcinoma Recommended Negative Controls: Mouse IgM Negative Control Applications: Immunohistochemistry: Frozen, Paraffin. 1:1000 Western Blotting: 1:1000 Immunoprecipitation Optimal working dilutions to be determined by researcher. Immunohistochemistry: *This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Note: Applications are based on unconjugated antibody.