P1001-26E
p53, mixed clones (Tumor Suppressor Protein, Oncogene Protein)
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£863.00
- SKU:
- P1001-26E
- Reinheit:
- Purified by Protein A affinity chromatography from ascites.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human p53. MW Of Antigen: 53kD. Epitope: aa37-45 and aa20-25. Cellular Localization: Nuclear
- Klon:
- DO-7+BP53–12
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See P1001-26EX
- Weitere Details:
- p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunoprecipitation (Native and denatured): (Use Protein A; 2ug/mg protein lysate) Western Blot: 0.5-1ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minute at RT. Staining of formalin/paraffin tissues is ENHANCED by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min Optimal dilution determined by the researcher. Positive Control: MDA-231, SKBR-3 cells or Colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice