P1001-25H
p53 Binding Protein 1 (53BP1, 53-BP1, FLJ41424, MGC138366, p202, p53BP1, Tumor Protein 53 Binding Protein 1, Tumor Protein p53 Binding Protein 1, TP53BP1, Tumor Suppressor p53 Binding Protein 1)
Größe
£800.00
- SKU:
- P1001-25H
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1. UniProt Accession: Q12888
- Klon:
- 5H37 (BP13)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Weitere Details:
- 53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. Small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrated that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-4ug/ml detected 53BP1 in lysates from HeLa cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate 53BP1 from HeLa cell lysate. Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for 53BP1 in HeLa cells fixed with 3% paraformaldehyde and permeabilized in 0.5% Triton X-100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice