P1001-25G
p53 Binding Protein 1 (53BP1, 53-BP1, FLJ41424, MGC138366, p202, p53BP1, Tumor Protein 53 Binding Protein 1, Tumor Protein p53 Binding Protein 1, TP53BP1, Tumor Suppressor p53 Binding Protein 1)
Größe
£800.00
- SKU:
- P1001-25G
- Reinheit:
- Ascites
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Mix of three GST fusion proteins corresponding to residues 1-337, 338-671 and 1331-1664, respectively, of human 53BP1.
- Klon:
- 5H36 (BP18)
- Format:
- Supplied as liquid, 0.05% sodium azide, 30% glycerol.
- Weitere Details:
- 53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. Small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrated that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage. Applications: Suitable for use in Western Blot and Immunoprecipitation(1). Other applications not tested. Recommended Dilution: Western Blot: 1:5000 -1:50,000 detected 53BP1 in whole cell lysates from HeLa cells. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1905-28 IgM (HRP) Pab GtxMo I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo I1905-28R IgM (mu chain specific) (HRP) Pab GtxMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice