P1001-23B

p45 (SKP2)

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£850.00

SKU:: P1001-23B
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Full-length recombinant protein corresponding to human p45 (SKP2).
Klon:: 3G225
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Weitere Details:: The F-box protein p45SKP2 is the substrate-targeting subunit of the protein ligase SCFSKP2 and is frequently overexpressed in transformed cells. Expression of p45SKP2 in quiescent fibroblasts promotes p27Kip1 degradation, allows the generation of cyclin-A-dependent kinase activity, and allows entry into the S phase of the cell cycle. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence/Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 1:250-1:500 Immunoprecipitation: 5ug Immunofluorescence (IC): 1:250 Optimal dilutions to be determined by the researcher. Positive Control: HeLa, Jurkat and 293T cell lines. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.