P1000-49A

p21Waf1 (Activating Fragment 1, p21CIP1, p21-CIP1, PIC1, SDI1, Wildtype p53 Activating Fragment 1, WAF1)

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£550.00

SKU:: P1000-49A
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Human p21 protein.
Klon:: 9L524 (WA-1)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Weitere Details:: The tumor suppressor p21Waf1 (wild-type p53-activated fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities. Applications: Suitable for use in ELISA, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Paraffin using human colon carcinoma. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.