P0999-60B

p15INK4b (Cyclin-dependent Kinase 4 Inhibitor B, Multiple Tumor Suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2)

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£825.00

SKU:: P0999-60B
Reinheit:: Purified by Protein A affinity chromatography from hybridoma culture supernatant
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to Arg2-Asp138 from human p15INK4b expressed in E.coli.
Klon:: 12K152
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: p15INK4B is an ~15kD member of the p16INK4 family. Human p15INK4B is 138aa in length and contains four ankyrin repeats, a common structural sequence found in members of the INK4 family. The first 50aa of p15INK4B share 44% sequence identity with p16INK4. Following this is an 81aa region sharing 97% sequence identity, after which the two proteins diverge. Human p15INK4B shares 83% aa sequence identity with mouse and rat p15INK4B. Functionally, p15INK4B interacts strongly with CDK4 and CDK6 and is a potent inhibitor. p15INK4B can potentially induce cell cycle arrest by acting as an effector of TGF-B. Applications: Suitable for use in Direct ELISA,, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using 293T human embryonic cell line and Hela human cervical epithelial carcinoma cell line Immunocytochemistry: 3-25ug/ml using immersion fixed HeLa human cervical epithelial carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice