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O8075-07

Ovalbumin

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£927.00

SKU:
O8075-07
Reinheit:
Ascites
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Chicken Egg Ovalbumin.
Klon:
7C41
Format:
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Weitere Details:
Chicken egg albumin (Ovalbumin) is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7kD. This sequence completely agrees with the reported m- RNA (messenger RNA) sequence. The carbohydrate and phosphate portions account for an additional 1428 and 160 grams per mole respectively, giving a total molecular weight of 44.3kD. Differential scanning calorimetry indicates the chicken egg albumin denatures at 84°C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp and 33 Glu which make it suitable for conjugation. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:5000 using ECL. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice