O6020-21

Oligo, Reverse Sequencing Primer M13 (19-mer), [5’-GGA AAC AGC TAT GAC CAT G-3’]

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£208.00

SKU:: O6020-21
Reinheit:: Purified by HPLC. Quality Control: MALDI-TOF MS
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with sterile dH2O, TE or Tris buffer, pH 7.0.
Weitere Details:: Single-stranded oligonucleotide, chemically synthesized, suitable for DNA applications. This M13 primer is a reverse sequencing primer that enables sequencing inserts in M13mp and pUC vectors. Special Labels: Biotin, Fluorescein, and Phosphate modifications can be included at the 5' or 3' site. For special labels add -B, -F, -P and -5, -3 to the catalog number. Sequence: 5' GGA AAC AGC TAT GAC CAT G 3' Molecular Weight: 5845.9g/mol GC Content: 47.4% Tm: 54.5°C Synthesis Scale: 50nmole Synthesis scale refers to the amount of the 3'-terminus base that the multi-step synthesis starts with. Final yield is indicated on the lot-specific certificate of analysis. Minimum Guaranteed Yield: 1.0 OD (A260nm) unit per mL reconstituted product 1 OD unit corresponds to ~33ug ssDNA Storage & Stability: Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.