Pou5f1 (POU Domain, Class 5, Transcription Factor 1, NF-A3, Octamer-binding Protein 3, Oct-3, Octamer-binding Protein 4, Oct-4, Octamer-binding Transcription Factor 3, OTF-3, Oct-3, Oct-4, Otf-3, Otf3)

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£642.00

SKU:: O5403-01X
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-DRPNAVKLEKVEP, from mouse OCT4, at the internal region of the protein (NP_038661.2). Species sequence homology: Rat.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml, observed in lysates of cell line NIH3T3 on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.