O5202
Occludin
Größe
£774.00
- SKU:
- O5202
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein consisting of the C-terminal region (~150aa) of human occludin. Species Sequence Homology: Bacteria, C.elegans, fish, guinea pig
- Klon:
- 3G217
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Weitere Details:
- Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1). Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Immunofluorescence (IC): 2-3ug/ml Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo Positive Control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice