N7100-61
NME1 (Nucleoside Diphosphate Kinase A, Granzyme A-activated DNase, GAAD, Metastasis Inhibition Factor nm23, NB, NBS, NDPKA, NDPK-A, NDP Kinase A, NM23, nm23-H1, NM23-H1, Tumor Metastatic Process-associated Protein)
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£812.00
- SKU:
- N7100-61
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant protein corresponding to Met1-Glu152 from human NME1, expressed in E. coli.
- Klon:
- 10K87
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- NME1 (NM23-H1) is a 19-20kD member of the NDK family of enzymes. NME1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NME2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NME1 is 152aa in length, contains one NDP kinase domain (aa5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NME1 shares 89% aa identity with human 17-18kD NME2, and 94% aa identity with mouse NME1. A second H1 isoform named M23-H1B with 25 additional aa at the N-terminus has also been described. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and K562 human chronic myelogenous leukemia cell line Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice