Nuclear Receptor Rev-ErbA alpha (Rev ErbA alpha, Rev-ErbA-alpha, Rev Erb alpha, Rev-Erb-alpha, Rev-erbalpha, Rev-ErbA, ErbA-related 1, EAR1, EAR-1, hRev, Nuclear Receptor Subfamily 1 Group D Member 1, NR1D1, Orphan Nuclear Receptor NR1D1, THRA1, Thyroid Hormone Receptor alpha-like, THRAL, V-erbA-related Protein EAR-1, V-erbA Related Protein EAR-1) (Biotin)

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£1,023.00

SKU:: N6889-85G-BIOTIN
Anwendung:: WB
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to residues surrounding the area near the N-terminus region of NR1D1. Epitope: Near N-terminus. Species sequence homology: mouse and rat
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Weitere Details:: Rev-ErbA alpha (NR1D1 or nuclear receptor subfamily 1, group D, member 1), is a member of the Rev-ErbA family of nuclear receptors and is a transcriptional repressor. In mammals, Rev-Erba is highly expressed in the liver, skeletal muscle, adipose tissue, and the brain, participating in the development and circadian regulation of these tissues. Rev-Erba regulates gene transcription by directly binding to target response elements (RevREs), comprises an A/T-rich flank followed by AGGTCA. Rev-Erba mediates repression by recruiting the corepressor N-CoR, which then activates the histone deacetylase (HDAC) 3. A number of target genes has been identified for Rev-Erba, including the lipoproteins ApoA1 and ApoCIII, hydratase dehydrogenase, the circadian factor BMAL, and the anti-fibrinolytic factor PAI-1. Many of these genes are coordinately regulated by Rev-erba and the RAR-related orphan receptor RORa, which share the same response elements but exert opposite effects on gene transcription. Crosstalk between Rev-Erba and RORa likely acts to fine-tune of their target physiologic networks, such as circadian rhythms, metabolic homeostasis, and inflammation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: A431 cell lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice