N6000-27
NTH (Endonuclease III Like 1, NTHL1, OCTS3) (Not for Export EU)
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£581.00
- SKU:
- N6000-27
- Reinheit:
- Serum
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full-length recombinant protein (human)
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Weitere Details:
- Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations. Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, a number of other damaged DNA sites are created by free-radical attack on DNA. The human NTH1 repair protein is one enzyme that has been shown to act on a large number of these other oxidatively damaged DNA sites (2). A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity. The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA (3). Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000-1:5,000 (ECL), band at ~37kD Immunocytochemistry/Immunofluorescence: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice