N5380-05

NR1, CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1)

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£940.00

SKU:: N5380-05
Reinheit:: Purified by Protein G affinity chromatography from ascites.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: His tag fusion protein corresponding to aa834-938 of rat NR1. Peptide sequence homology: human and mouse, 100%
Klon:: 0.T.190
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Weitere Details:: NR1 along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. Applications: Suitable for use in Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: Shows positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons. Immunoprecipitation: 4ug immunoprecipitated NR1 from 500ug of rat brain microsomal protein preparation. Western Blot: 0.5-2ug/ml. Detects NR1 from 20ug of rat brain microsomal protein preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with N5380-05 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Note: Do not boil the microsomal preparation. Incubate at RT for 30-45 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Brain tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice