N5375-51B
Notch 4, ID (Notch Homolog 4, Drosophila, INT3)
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£800.00
- SKU:
- N5375-51B
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- E. coli-derived recombinant human Notch4 Intracellular Domain (aa1778-2003) Accession # Q99466.
- Klon:
- 7H240
- Format:
- Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
- Weitere Details:
- Notch4 is a 250kD member of the NOTCH family of proteins. It is primarily expressed on endothelium. Human Notch4 is a 1980aa type I transmembrane (TM) glycoprotein that contains a 1424 aa extracellular domain (ECD) and a 535aa cytoplasmic region. Notch4 undergoes proteolytic processing at multiple sites. There is probable cleavage of the ECD by furin in the Golgi between aa1310-1410. This creates a mature, potentially disulfide-linked heterodimer at the cell surface. Upon ligand binding, two sequential cleavages occur, one in the ECD after Ala1431, and a second in the TM domain after Lys1466. This creates a soluble cytoplasmic fragment that interacts with RBP-Jκ and activates genes associated with HES-1. Over cytoplasmic aa1778-2003, human Notch-4 is 78% aa identical tomouse Notch4. Applications: Suitable for use in Immunocytochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry: 10ug/ml ELISA (Direct): 0.5-1ug/ml. Detection limit ~2ng/well. Western Blot: 1-2ug/ml. Detection limit ~5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice