N5375-26E
Notch3 (Notch Homolog 3 (Drosophila), hN3, Neurogenic Locus Notch Homolog Protein 3, CADASIL, CASIL) (APC)
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£842.00
- SKU:
- N5375-26E
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by immunoaffinity chromatography
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ala40-Glu467 from human Notch-3, expressed in NS0 cells.
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early event developmental processes.1 The extracellular domains of Notch receptors interact with the extracellular domains of the transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. Notch-3 has the same biochemical mechanism of signal transduction as Notch-1, where a series of cleavage events release of the Notch intracellular domain (NICD). NICD translocates to the nucleus and initiates transcription of Notch responsive genes.2 Thus, Notch acts as both a ligand binding receptor and a nuclear factor that regulates transcription. Human Notch-3 shows 90% aa identity to mouse Notch-3 over the entire protein. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Dry Ice