N5375-17H
Notch 2 (Notch2, Notch-2, Neurogenic Locus Notch Homolog Protein 2 Precursor, hN2, Aicardi-Goutieres Syndrome 2, AGS2)
Größe
£385.00
- SKU:
- N5375-17H
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding residues surrounding Val2332 of human Notch2.
- Klon:
- 11C1055(D67C8)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:400 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice