Notch1 (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1) (Not for Export EU)

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£557.00

SKU:: N5375-08
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide CQHSYSSPVDNTPSHQ corresponding to amino acid residues 2488-2502 of human Notch 1, intracellular domain. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Format:: Supplied as liquid in PBS, 0.01% sodium azide. No stabilizing proteins added.
Weitere Details:: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Applications: Suitable for use in, ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:20,000-1:100,000 Western Blot: 1:1000-1:10,000 Immunohistochemistry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice