N5375-03K

Notch-1 (hN1, LIN12, Lin-12, MIS6, Neurogenic Locus Notch Homolog Protein 1, NOTC1, Notch (Drosophila) Homolog 1 (Translocation Associated), Notch 1 Extracellular Truncation, Notch 1 Intracellular Domain, Notch Homolog 1, Notch Homolog 1 Translocation Associated (Drosophila), Translocation Associated (Drosophila), Translocation Associated Notch Homolog, Translocation Associated Notch Protein TAN1)

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£825.00

SKU:: N5375-03K
Reinheit:: Purified by Protein G affinity chromatography from cell culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corrsponding to aa19-526 from human Notch-1 expressed in NSO cells.
Klon:: 9H166
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: Notch-1 (so named for “notches” in fly wings; also TAN-1) is a 300kD, 2538aa member of the notch family of type I TM glycoproteins. It is associated with gene activation in both embryo and adult. It undergoes Golgi processing to generate a heterodimer composed of a 180-200kD disulfide-linked extracellular domain (aa18-1664), and a 120kD membrane-bound segment (aa 1665-2556) that is cleaved to form the notch intracellular domain transcription factor upon ligand binding. Human Notch-1 ECD aa19-526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat Notch-1, and 60% aa identity with human Notch-2 and Notch-3. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells using U2OS human osteosarcoma cell line. ELISA (Capture): 2-8ug/ml, used with N5375-03Z as the detection antibody Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice